Review



mouse anti eea1  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Cell Signaling Technology Inc mouse anti eea1
    Mouse Anti Eea1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+eea1/EEA1+Mouse+mAb/pmc13015250-12-0-2
    Average 95 stars, based on 56 article reviews
    mouse anti eea1 - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: Rab9 depletion enhances human adenovirus type 26 transduction efficiency through increased internalization and reduced late endosomal/lysosomal retention
    Article Snippet: .. Primary antibodies used for immunofluorescence and co-localization analyses, or western blot were as follows: anti-EEA1 (#2411, Cell Signaling, SAD), anti-LAMP1 (ab24170, Abcam, UK), anti-Rab5 (#3547, Cell Signaling, SAD), anti-Rab7 (#9367, Cell Signaling, SAD), anti-Rab9A (#5118, Cell Signaling, SAD), anti-Alexa-Fluor-488 recombinant F(ab)-rabbit monoclonal antibody (15H9L93, Invitrogen). ..

    Article Title: Enucleated cells with Nectin-1 overexpression capture HSV-1 and promote viral elimination for herpes simplex encephalitis therapy.
    Article Snippet: .. Immunofluorescence staining was performed using anti-RCAS1 (Cell Signaling Technology, 12290); anti-EEA1 (Cell Signaling Technology, 3288); anti-PDI (Cell Signaling Technology, 3501) and anti-LAMP1 (Cell Signaling Technology, 99437) as primary antibodies. ..

    Article Title: A proximity map of RAB GTPases delineates roles for RAB14 in EARP complex and UHRF1BP1 endosomal recruitments.
    Article Snippet: .. Cells were fixed and immunofluorescence analysis was performed as described above.Antibodiesused throughout the studywere anti-MYC(1:500; 71D10; CST), anti-transferrin receptor (1:100; D59X; CST), anti-EEA1 (1:500; C45B10; CST), anti-HA (1:500; C29F4; CST), anti-HA (1/200; F-7; Santa Cruz Biotechnology), anti-TGN46 (1:100; Clone 2F7.1; Genetex), antiCIMPR (1:100; MCA2048; BioRad), anti-LAMP1 (1:15; 1D4B; DHSB), anti-KDEL (1:200; EPR12668;Abcam), anti-TOM20 (1:200; 29/Tom20;BD Biosciences), anti-active β1-integrin (1:100; 12G10; Millipore Sigma), antiRAB11 (1/50; D4F5; CST), anti-RAB11 (1/100; 610656; BD Biosciences), anti-GM130 (1/3000; D6B1; CST), anti-CD63 (1/300; H5C6; BD Biosciences), anti-RAB14 (1/50; HPA026419; Millipore Sigma). .. Cells were mounted on slides in 4 μL of slow fade gold mounting media containing DAPI (S36938; Invitrogen (Thermo Fischer Scientific)).

    Western Blot:

    Article Title: Rab9 depletion enhances human adenovirus type 26 transduction efficiency through increased internalization and reduced late endosomal/lysosomal retention
    Article Snippet: .. Primary antibodies used for immunofluorescence and co-localization analyses, or western blot were as follows: anti-EEA1 (#2411, Cell Signaling, SAD), anti-LAMP1 (ab24170, Abcam, UK), anti-Rab5 (#3547, Cell Signaling, SAD), anti-Rab7 (#9367, Cell Signaling, SAD), anti-Rab9A (#5118, Cell Signaling, SAD), anti-Alexa-Fluor-488 recombinant F(ab)-rabbit monoclonal antibody (15H9L93, Invitrogen). ..

    Recombinant:

    Article Title: Rab9 depletion enhances human adenovirus type 26 transduction efficiency through increased internalization and reduced late endosomal/lysosomal retention
    Article Snippet: .. Primary antibodies used for immunofluorescence and co-localization analyses, or western blot were as follows: anti-EEA1 (#2411, Cell Signaling, SAD), anti-LAMP1 (ab24170, Abcam, UK), anti-Rab5 (#3547, Cell Signaling, SAD), anti-Rab7 (#9367, Cell Signaling, SAD), anti-Rab9A (#5118, Cell Signaling, SAD), anti-Alexa-Fluor-488 recombinant F(ab)-rabbit monoclonal antibody (15H9L93, Invitrogen). ..

    Staining:

    Article Title: Enucleated cells with Nectin-1 overexpression capture HSV-1 and promote viral elimination for herpes simplex encephalitis therapy.
    Article Snippet: .. Immunofluorescence staining was performed using anti-RCAS1 (Cell Signaling Technology, 12290); anti-EEA1 (Cell Signaling Technology, 3288); anti-PDI (Cell Signaling Technology, 3501) and anti-LAMP1 (Cell Signaling Technology, 99437) as primary antibodies. ..

    Blocking Assay:

    Article Title: Cholesterol Deficiency Directs Autophagy‐Dependent Secretion of Extracellular Vesicles
    Article Snippet: Before antibody staining, cells were fixed in 4% paraformaldehyde (PFA; Electron Microscopy Sciences, 15714) at room temperature for 20 min. Next, cells were permeabilized with 0.2% Triton X‐100 (Sigma‐Aldrich, 93443) for 20 min and then incubated in blocking buffer (5% donkey or goat serum, Jackson ImmunoResearch Laboratories, 017‐000‐121 or 005‐000‐121; 0.1% Triton X‐100) for 1 h at room temperature. .. The primary antibodies used for these studies were diluted in blocking buffer and included rabbit anti‐Rab5 (Cell Signaling Technology, 3547, 1:200), rabbit anti‐EEA1 (Cell Signaling Technology, 3288, 1:200), rabbit anti‐Rab7 (Cell Signaling Technology, 9367, 1:200), rabbit anti‐Rab11a (Cell Signaling Technology, 5589, 1:200), mouse anti‐p62 (Cell Signaling Technology, 88588, 1:200), rabbit anti‐LC3B (Cell Signaling Technology, 3868, 1:200), rabbit anti‐LAMP1 (Novus, 19294, 1:1000) and mouse anti‐CD63 (Novus, 77913, 1:500). .. Primary antibodies were visualized with Alexa Fluor‐conjugated secondary antibodies (Life Technologies, A11001, A21437 and A31570 ; 1:500).



    Similar Products

    95
    Cell Signaling Technology Inc mouse anti eea1
    Mouse Anti Eea1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+eea1/EEA1+Mouse+mAb/pmc13015250-12-0-2
    Average 95 stars, based on 1 article reviews
    mouse anti eea1 - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    93
    Cell Signaling Technology Inc eea1 2411s
    Enhanced recruitment of Rab5 to lipid droplets (LDs) upon lipophagy stimulation . A , Western blot analysis of Rab5 and <t>EEA1</t> GTP-pulldown in regular medium (CT) or 4 h HBSS starvation in Hep3B cells. B , quantification of the fold change of GTP-bound/total Rab5 as well as EEA1 from n = 4 independent experiments. C , quantification of total Rab5–β-actin confirming that starvation does not change overall Rab5 expression. D , Western blot analysis of LDs isolated from Hep3B cells by density gradient centrifugation showing abundant levels of Rab5 in isolated LDs following 4 h of HBSS starvation compared with the regular medium (CT). E , quantification of Rab5–Plin2 in the biochemically isolated LDs. F , fluorescence micrograph showing immunofluorescence staining of Hep3B cells expressing Myc-tagged Rab5 ( green ). Cells were treated with oleic acid for 16 h and then either maintained in regular medium (control) or subjected to 4 h of HBSS starvation before being fixed and stained with Oil Red O (ORO; red ) to label LDs. Note the marked increase in Rab5 staining around LDs in HBSS-starved cells versus control ( yellow arrowheads ). Graphs represent mean ± SD from n = 3 to 4 independent experiments. Statistical significance was determined using an unpaired two-tailed t test. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. CT, control; EEA1, early endosome antigen 1; HBSS, Hank’s balanced salt solution; Plin2, perilipin-2.
    Eea1 2411s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+eea1/Rab5A+Antibody/pmc13010930-197-7-12
    Average 93 stars, based on 1 article reviews
    eea1 2411s - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc eea1
    Internalization of cetuximab-vc-MMAF. A. OVCAR8 cells were treated with 10 nM cetuximab-vc-MMAF (red) at 37°C for the indicated times. For time 0 h, cells were incubated on ice for 30 minutes to visualize membrane staining. Nuclei were stained with Hoechst 33342 in all cases. Scale bar: 10 μm. B. Colocalization of cetuximab-vc-MMAF (red) with endocytic markers (green). Cells were treated as above, and colocalization (yellow) with markers for early endosomes <t>(EEA1),</t> late endosomes (RAB7), and lysosomes (LAMP1) analysed at the indicated times. Scale bar: 5 μm. C. Quantitation of colocalization was performed using ImageJ and expressed as a percentage. Data are presented as the mean ± SD of 10 fields per time point, for each vesicle type.
    Eea1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+eea1/pmc12997193-53-4-16
    Average 86 stars, based on 1 article reviews
    eea1 - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    95
    Proteintech anti eea1
    Internalization of cetuximab-vc-MMAF. A. OVCAR8 cells were treated with 10 nM cetuximab-vc-MMAF (red) at 37°C for the indicated times. For time 0 h, cells were incubated on ice for 30 minutes to visualize membrane staining. Nuclei were stained with Hoechst 33342 in all cases. Scale bar: 10 μm. B. Colocalization of cetuximab-vc-MMAF (red) with endocytic markers (green). Cells were treated as above, and colocalization (yellow) with markers for early endosomes <t>(EEA1),</t> late endosomes (RAB7), and lysosomes (LAMP1) analysed at the indicated times. Scale bar: 5 μm. C. Quantitation of colocalization was performed using ImageJ and expressed as a percentage. Data are presented as the mean ± SD of 10 fields per time point, for each vesicle type.
    Anti Eea1, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+eea1/EEA1+Antibody/pmc13025052-241-29-31
    Average 95 stars, based on 1 article reviews
    anti eea1 - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc antibodies against eea1
    Internalization of cetuximab-vc-MMAF. A. OVCAR8 cells were treated with 10 nM cetuximab-vc-MMAF (red) at 37°C for the indicated times. For time 0 h, cells were incubated on ice for 30 minutes to visualize membrane staining. Nuclei were stained with Hoechst 33342 in all cases. Scale bar: 10 μm. B. Colocalization of cetuximab-vc-MMAF (red) with endocytic markers (green). Cells were treated as above, and colocalization (yellow) with markers for early endosomes <t>(EEA1),</t> late endosomes (RAB7), and lysosomes (LAMP1) analysed at the indicated times. Scale bar: 5 μm. C. Quantitation of colocalization was performed using ImageJ and expressed as a percentage. Data are presented as the mean ± SD of 10 fields per time point, for each vesicle type.
    Antibodies Against Eea1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+eea1/EEA1+Antibody/pm41850405-130-6-13
    Average 96 stars, based on 1 article reviews
    antibodies against eea1 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc 355 369 anti eea1 rabbit mab if
    Internalization of cetuximab-vc-MMAF. A. OVCAR8 cells were treated with 10 nM cetuximab-vc-MMAF (red) at 37°C for the indicated times. For time 0 h, cells were incubated on ice for 30 minutes to visualize membrane staining. Nuclei were stained with Hoechst 33342 in all cases. Scale bar: 10 μm. B. Colocalization of cetuximab-vc-MMAF (red) with endocytic markers (green). Cells were treated as above, and colocalization (yellow) with markers for early endosomes <t>(EEA1),</t> late endosomes (RAB7), and lysosomes (LAMP1) analysed at the indicated times. Scale bar: 5 μm. C. Quantitation of colocalization was performed using ImageJ and expressed as a percentage. Data are presented as the mean ± SD of 10 fields per time point, for each vesicle type.
    355 369 Anti Eea1 Rabbit Mab If, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+eea1/EEA1+Rabbit+mAb/pm41833373-351-4-10
    Average 96 stars, based on 1 article reviews
    355 369 anti eea1 rabbit mab if - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc anti eea1
    Internalization of cetuximab-vc-MMAF. A. OVCAR8 cells were treated with 10 nM cetuximab-vc-MMAF (red) at 37°C for the indicated times. For time 0 h, cells were incubated on ice for 30 minutes to visualize membrane staining. Nuclei were stained with Hoechst 33342 in all cases. Scale bar: 10 μm. B. Colocalization of cetuximab-vc-MMAF (red) with endocytic markers (green). Cells were treated as above, and colocalization (yellow) with markers for early endosomes <t>(EEA1),</t> late endosomes (RAB7), and lysosomes (LAMP1) analysed at the indicated times. Scale bar: 5 μm. C. Quantitation of colocalization was performed using ImageJ and expressed as a percentage. Data are presented as the mean ± SD of 10 fields per time point, for each vesicle type.
    Anti Eea1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+eea1/EEA1+Rabbit+mAb/pm41824450-580-10-11
    Average 96 stars, based on 1 article reviews
    anti eea1 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results


    Enhanced recruitment of Rab5 to lipid droplets (LDs) upon lipophagy stimulation . A , Western blot analysis of Rab5 and EEA1 GTP-pulldown in regular medium (CT) or 4 h HBSS starvation in Hep3B cells. B , quantification of the fold change of GTP-bound/total Rab5 as well as EEA1 from n = 4 independent experiments. C , quantification of total Rab5–β-actin confirming that starvation does not change overall Rab5 expression. D , Western blot analysis of LDs isolated from Hep3B cells by density gradient centrifugation showing abundant levels of Rab5 in isolated LDs following 4 h of HBSS starvation compared with the regular medium (CT). E , quantification of Rab5–Plin2 in the biochemically isolated LDs. F , fluorescence micrograph showing immunofluorescence staining of Hep3B cells expressing Myc-tagged Rab5 ( green ). Cells were treated with oleic acid for 16 h and then either maintained in regular medium (control) or subjected to 4 h of HBSS starvation before being fixed and stained with Oil Red O (ORO; red ) to label LDs. Note the marked increase in Rab5 staining around LDs in HBSS-starved cells versus control ( yellow arrowheads ). Graphs represent mean ± SD from n = 3 to 4 independent experiments. Statistical significance was determined using an unpaired two-tailed t test. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. CT, control; EEA1, early endosome antigen 1; HBSS, Hank’s balanced salt solution; Plin2, perilipin-2.

    Journal: The Journal of Biological Chemistry

    Article Title: Rab5 nucleotide binding promotes oxidative metabolism to fuel hepatocellular carcinoma cell proliferation

    doi: 10.1016/j.jbc.2026.111321

    Figure Lengend Snippet: Enhanced recruitment of Rab5 to lipid droplets (LDs) upon lipophagy stimulation . A , Western blot analysis of Rab5 and EEA1 GTP-pulldown in regular medium (CT) or 4 h HBSS starvation in Hep3B cells. B , quantification of the fold change of GTP-bound/total Rab5 as well as EEA1 from n = 4 independent experiments. C , quantification of total Rab5–β-actin confirming that starvation does not change overall Rab5 expression. D , Western blot analysis of LDs isolated from Hep3B cells by density gradient centrifugation showing abundant levels of Rab5 in isolated LDs following 4 h of HBSS starvation compared with the regular medium (CT). E , quantification of Rab5–Plin2 in the biochemically isolated LDs. F , fluorescence micrograph showing immunofluorescence staining of Hep3B cells expressing Myc-tagged Rab5 ( green ). Cells were treated with oleic acid for 16 h and then either maintained in regular medium (control) or subjected to 4 h of HBSS starvation before being fixed and stained with Oil Red O (ORO; red ) to label LDs. Note the marked increase in Rab5 staining around LDs in HBSS-starved cells versus control ( yellow arrowheads ). Graphs represent mean ± SD from n = 3 to 4 independent experiments. Statistical significance was determined using an unpaired two-tailed t test. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. CT, control; EEA1, early endosome antigen 1; HBSS, Hank’s balanced salt solution; Plin2, perilipin-2.

    Article Snippet: Antibodies against Rab5A (2143S), PLIN2 (95109), and EEA1 (2411S) were purchased from Cell Signaling Technology.

    Techniques: Western Blot, Expressing, Isolation, Gradient Centrifugation, Fluorescence, Immunofluorescence, Staining, Control, Two Tailed Test

    Inhibition of Rab5 GTPase activity reduces lipid droplet (LD) catabolism via its GTPase activity . A , Western blot analysis of Rab5 and EEA1 GTP-pulldown in Hep3B cells treated with DMSO (CT) or NAP (100 μM, 48 h). B , quantification of GTP/total Rab5 as well as EEA1 from n = 3 independent experiments. C , confocal micrograph showing ORO-stained LDs ( red ) and DAPI-stained nuclei from Hep3B cells treated with DMSO (control) and NAP (100 μM). D , bar graphs depict quantification of LD number/cell, total LD area/cell, and LD size. E , confocal micrograph showing ORO-stained LDs (red ) and DAPI-stained nucleus from Hep3B cells treated with DMSO (control) and NAP (100 μM) with oleic acid (OA, 150 μM). F , bar graphs depict quantification of LD number/cell, total LD area/cell, and LD size. G , cartoon depicting NAP alteration of Rab5 GTP binding. Graphs represent mean ± SD from n = 3 independent experiments. Statistical significance was determined using an unpaired two-tailed t test. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. CT, control; DAPI, 4′,6-diamidino-2-phenylindole; DMSO, dimethyl sulfoxide; EEA1, early endosome antigen 1; NAP, neoandrographolide; ORO, Oil Red O.

    Journal: The Journal of Biological Chemistry

    Article Title: Rab5 nucleotide binding promotes oxidative metabolism to fuel hepatocellular carcinoma cell proliferation

    doi: 10.1016/j.jbc.2026.111321

    Figure Lengend Snippet: Inhibition of Rab5 GTPase activity reduces lipid droplet (LD) catabolism via its GTPase activity . A , Western blot analysis of Rab5 and EEA1 GTP-pulldown in Hep3B cells treated with DMSO (CT) or NAP (100 μM, 48 h). B , quantification of GTP/total Rab5 as well as EEA1 from n = 3 independent experiments. C , confocal micrograph showing ORO-stained LDs ( red ) and DAPI-stained nuclei from Hep3B cells treated with DMSO (control) and NAP (100 μM). D , bar graphs depict quantification of LD number/cell, total LD area/cell, and LD size. E , confocal micrograph showing ORO-stained LDs (red ) and DAPI-stained nucleus from Hep3B cells treated with DMSO (control) and NAP (100 μM) with oleic acid (OA, 150 μM). F , bar graphs depict quantification of LD number/cell, total LD area/cell, and LD size. G , cartoon depicting NAP alteration of Rab5 GTP binding. Graphs represent mean ± SD from n = 3 independent experiments. Statistical significance was determined using an unpaired two-tailed t test. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. CT, control; DAPI, 4′,6-diamidino-2-phenylindole; DMSO, dimethyl sulfoxide; EEA1, early endosome antigen 1; NAP, neoandrographolide; ORO, Oil Red O.

    Article Snippet: Antibodies against Rab5A (2143S), PLIN2 (95109), and EEA1 (2411S) were purchased from Cell Signaling Technology.

    Techniques: Inhibition, Activity Assay, Western Blot, Staining, Control, Binding Assay, Two Tailed Test

    Internalization of cetuximab-vc-MMAF. A. OVCAR8 cells were treated with 10 nM cetuximab-vc-MMAF (red) at 37°C for the indicated times. For time 0 h, cells were incubated on ice for 30 minutes to visualize membrane staining. Nuclei were stained with Hoechst 33342 in all cases. Scale bar: 10 μm. B. Colocalization of cetuximab-vc-MMAF (red) with endocytic markers (green). Cells were treated as above, and colocalization (yellow) with markers for early endosomes (EEA1), late endosomes (RAB7), and lysosomes (LAMP1) analysed at the indicated times. Scale bar: 5 μm. C. Quantitation of colocalization was performed using ImageJ and expressed as a percentage. Data are presented as the mean ± SD of 10 fields per time point, for each vesicle type.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Rational payload selection enables high antitumoral efficacy of an anti-EGFR antibody-drug conjugate against ovarian tumors

    doi: 10.1016/j.neo.2026.101295

    Figure Lengend Snippet: Internalization of cetuximab-vc-MMAF. A. OVCAR8 cells were treated with 10 nM cetuximab-vc-MMAF (red) at 37°C for the indicated times. For time 0 h, cells were incubated on ice for 30 minutes to visualize membrane staining. Nuclei were stained with Hoechst 33342 in all cases. Scale bar: 10 μm. B. Colocalization of cetuximab-vc-MMAF (red) with endocytic markers (green). Cells were treated as above, and colocalization (yellow) with markers for early endosomes (EEA1), late endosomes (RAB7), and lysosomes (LAMP1) analysed at the indicated times. Scale bar: 5 μm. C. Quantitation of colocalization was performed using ImageJ and expressed as a percentage. Data are presented as the mean ± SD of 10 fields per time point, for each vesicle type.

    Article Snippet: The antibodies directed to EEA1, RAB7, LAMP1, β-tubulin, cleaved caspase-3, pRb (Ser780), and pH2AX were from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Incubation, Membrane, Staining, Quantitation Assay